Part:BBa_T2013:Design

Strep-tag II head domain (WSHPQFEK)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This domain encodes the Strep-tag II sequence WSHPQFEK. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

An ATG start codon was used followed by a AAA second codon. AAA is the preferred codon for the second codon position in E. coli.

Source

This is a synthetic sequence.

References

<biblio>

1. Schmidt93 pmid=8433964

// this looks to be the original reference for the Strep-tag

1. Skerra96 pmid=8636976

// original reference for Strep-tag II

1. Voss97 pmid=9415448

// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.

1. Skerra99 pmid=10796988
2. Skerra00 pmid=11036648

// history of Strep-tag system

1. Skerra07 pmid=17571060

// includes brief overview of Strep-tag system </biblio>